National College Rawalpindi

Microtechniques: chapter 10 introduction to microtechniques

Microtechniques refer to specialized laboratory methods used to prepare biological specimens for microscopic examination. These techniques are essential in fields such as biology, histology, pathology, and medical research, where studying tissues and cells at the microscopic level is crucial. The main steps in microtechniques typically include fixation (preserving tissue structure), dehydration (removing water using alcohol), embedding (usually in paraffin wax), sectioning (cutting thin slices using a microtome), and staining (enhancing contrast with dyes). Each step ensures that the tissue retains its natural form and can be clearly viewed under a microscope. Microtechniques help scientists and medical professionals diagnose diseases, study cell structure, and conduct research. Different staining methods, like Hematoxylin and Eosin (H&E), are used to highlight specific parts of the cells or tissues. The accuracy and quality of these procedures are vital for correct analysis. Overall, microtechniques play a foundational role in modern biological and medical sciences Topic: Introduction & Techniques


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